Comparative Study of Melatonin Versus Infliximab on ZnO-NPs-Induced Pancreatic Injury in Adult Male Albino Rats: A Light and Electron Microscope Study.

Articles in Press

Document Type : Original Article

Authors

1 histology, faculty of medicine, menoufia

2 Histology and cell biology department , faculty of medicine, menofia university

3 histology department.faculty of medicine,menuofiya university

4 histology and cell biology -faculty of medicine menoufia university

Abstract

ABSTRACT
Background
Increasingly significant attention is paid to zinc oxide nanoparticles (ZnO-NPs) in biomedicine and bioengineering. Despite their advantages, numerous studies have shown their health risks. Infliximab and melatonin exhibit anti-inflammatory and antioxidant properties.
Aim
To compare the protective benefits of melatonin versus infliximab against ZnO-NPs-induced pancreatic histological damage in adult male albino rats.
Materials and Methods
Thirty-six albino rats were divided into four groups: Group I (control, n=6), Group II (melatonin-treated, n=5 at a dose of (20 mg/kg/day) intraperitoneally (IP) every day for two weeks, Group III (infliximab-treated, n=5) at a dose of (5 mg/kg/week) IP once a week for two weeks, Group IV (ZnO-NPs-treated, n=20) at a dose of (250 mg/kg) IP. The experiment lasted two weeks, except with Group IV which was subdivided into subgroups IVa (melatonin, n=10) and IVb (infliximab, n=10). They were treated as Groups II and III for an additional two weeks. By the end of the experiment, the rats were anesthetized, and blood samples were withdrawn for biochemical analysis, then pancreatic specimens were processed for histological, histochemical and ultrastructural examination.
Results
ZnO-NPs-treated rats showed degenerative histological changes with increased collagen deposition in the pancreas. Immunohistochemically, there was strong positive caspase 3, TNF, and VEGF immunoreactivity and weak anti-insulin immunoreactivity. Administration of melatonin or infliximab ameliorated these changes. Light and electron microscopy findings were consistent.
Conclusion
The pancreas structure was severely damaged by ZnO-NPs. Melatonin treatment resulted in a partial improvement of these hazardous effects, and infliximab effectively reduced them. Hence, it is recommended to give infliximab and melatonin to people subjected to ZnO-NPs.

Keywords

Egyptian Journal of Histology

Articles in Press, Accepted Manuscript
Available Online from 12 March 2025

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