Gene Expression Analysis and Anti-cancer Activity of Cyclophosphamide for MCF-7 and H9 Cancer Cell Lines

Habeeb, Aymen Radhi; AL-tameemi, Hamid; Hussein, Talib Waseen

doi:10.21608/ejh.2024.313634.2125

Gene Expression Analysis and Anti-cancer Activity of Cyclophosphamide for MCF-7 and H9 Cancer Cell Lines

Document Type : Original Article

Authors

¹ Directorate of education of Rasafa , Ministry of education, Iraq

² University of Bilad Alrafidain, Baqubah, Iraq

10.21608/ejh.2024.313634.2125

Abstract

Introduction: Cancer is a major health problem worldwide, and it is one of the leading causes of death. Preventing tumor development is a major challenge due to the similarity in cellular mechanisms between cancerous and normal cells; however, when using biological substances have high efficiency against cancerous cells and low toxicity against normal cells associated with a good prognosis. Therefore, the current study is designed to study the role of Anise extract (AE) and Anise extract- Chitosan nanoparticles in the synergistic effect with cisplatin in treatment of tumor-induced in female mice.
Materials and Methods: Five groups of female mice, 6 mice for each group were included to achieve the goal of the present study. These study groups were control, tumor-induced, Cisplatin treated, Cisplatin – Anise extract treated, and Cisplatin- Anise extract- Chitosan nanoparticles treated groups. The tumor size and some biomarkers (IL-33, LRG-1, and CRP) were considered as indicators for tumor development and response to the treatment protocol.
Results: The present study found that the tumor size in the tumor-induced group reach to 33.1 mm after 30 days of inducement, while the tumor size in Cisplatin treated group was significantly smaller than the tumor-induced group. The tumor size in Cisplatin- Anise extract group was smaller than Cisplatin treated group. Also, the effect of cisplatin against a tumor increased when combining anise extract with Chitosan nanoparticles. Due to biomarkers showed IL-33, LRG-1, and CRP levels were higher in tumor group than control and other study groups.
Conclusion: The current study concluded that the anise extract has high effectiveness in reinforcement Cisplatin action against tumor development and at the same time increased their effect by nanoparticles.

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